Brand Name: SGONEK
Price: $270 -$308/Kilogram
Place of Origin: Shaanxi, China
Certification: ISO FDA SG COA MSDS
Grade: Food Grade, Medicine Grade, Tech Grade
Payment Terms: T/T, Western Union, MoneyGram
| Product Name: | Proteinase K | Cas No.: | 39450-01-6 |
| Molecular Formula: | C29H27N2O12P | Purity: | 99%min |
| Brand Name: | SGONEK | Certification: | ISO FDA SG COA MSDS |
| Product Name | Proteinase K |
| Purity | 99% |
| Cas No | 39450-01-6 |
| Appearance | White Powder |
| Synonyms | ProteinaseK,PowderAndSolutionAvailable;ProteinaseK,fromTritirachiumalbumLimber;Proteinase K from Tritirachium album ca. 8 DMC-U/mg;PROTEINASEK,LYOPHILIZEDPOWDER,BIOTECHGRADE;Proteinase K for tritirachium album serine.;proteinase k for tritirachium album;Protease K;Tritirachium album proteinase K |
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Descripition: Proteinase K is a stable and highly reactive serine protease. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp39-His69-Ser224). It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature. In the presence of 0.1-0.5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA. |
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|
Description |
White powder |
Complies |
|
Identification |
IR |
Complies |
|
|
HPLC |
Complies |
|
Heavy metal |
≤10ppm |
5ppm |
|
Pb |
≤3ppm |
1.5ppm |
|
Hg |
≤0.1ppm |
0.05ppm |
|
Cd |
≤1ppm |
0.2ppm |
|
Loss on drying |
≤0.5% |
0.12 |
|
Residue on lgnition |
≤0.1% |
0.03 |
|
Single impurity |
≤0.5% |
0.12 |
|
Total impurity |
≤1.0% |
0.29 |
|
Total bacteria |
≤1000cfu/g |
<1000 |
|
Yeast and Mould |
≤100 cfu/g |
<000 |
|
E.coli/25g |
Absent |
Absent |
|
Salmonella/25g |
Absent |
Absent |
|
Assay |
≥99.0% |
99.4% |
The application of this Mutated Proteinase K is similar with wild type Proteinase K. But the mutated one has higher specific activity and more stable at RT. It is a broad substrate non-specific serine Proteinase. It is active over the pH range 4-11. It was used on any situation to digest native and denatured proteins. For instance, it is used for isolating mRNA or genomic DNA from different tissues and modifying glycoprotein for structure studies.Mutated Proteinase K is active with SDS, urea and EDTA and active between the temperature15-75ºC.
Now, Proteinase K is a broad substrate non-specific serine proteinase. It was used on isolating mRNA, genomic DNA and digesting unwanted proteins during DNA and RNA preparations from different kinds of cells.
It’s been used on glycoprotein modification and protein structure studies also. Proteinase K is active with SDS, urea and EDTA.
Proteinase K is used for the isolation of native high molecular genomic nucleic acids. Enzymes like DNases and RNases from microorganisms and mammalian cells are rapidly inactivated by Proteinase K.
Adding Proteinase K already during the cell lysis enables the isolation of highly native undamaged high molecular DNA or RNA. A variety of methods have been established, which are documented in numerous publications.
